IKAN TRANSGENIK PDF

IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Jurnal Akuakultur Indonesia Vol 6, No 1 Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan tgansgenik faktor sigositas memberikan pengaruh nyata.

In this experiment, we applied a quantitative real-time PCR qr-PCR method to analyze zygosity in a stable line of transgenic zebrafish Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene.

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Jurnal Akuakultur Indonesia Publisher: The intensity and capacity of production is expected to increase using biotechnology approach. Now, a transgenic system to study the modification of fatty acid traansgenik pathways in fish was established.

This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact.

Pada transgeenik ini, kami menggunakan metode PCR real-time kuantitatif krt-PCR tramsgenik menganalisa sigositas pada satu strain ikan zebra Danio rerio transgenik yang membawa gen D6-desaturase-like dari ikan salmon masu, Oncorhynchus masou. This technique has been applied to several commercially valuable species. Transgenic individuals were identified by polymerase chain reaction PCR and their expression levels were analyzed by reverse transcription PCR.

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Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga.

Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel.

Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat kkan populasi manusia.

New approaches addressing this problem could be of great help for fish biotechnologists.

Recently, global food security has become a hot issue by the public in national as well as out of the country. Ioan method needs relatively large amounts of fish material and is time- and labor-intensive.

Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. Mating of those fish with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws.

This Author published in this journals. Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health and development. Data dianalisis menggunakan metode pembandingan nilai cycle threshold.

The transmission rate of transgene into F1 generation was 4. Jurnal Akuakultur Indonesia Vol 2, No 1 The first F1 and second F2 generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish.

Thus, we found that the qTR-PCR to kian effective for a rapid and precise determination of zygosity in transgenic fish. Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish.

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The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis. Data were analyzed using the comparative cycle threshold method. One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique.

This review describes various techniques of gene transggenik, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture. Rapid method for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti pola segregasi Mendel. Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti trnasgenik perikanan.

Identification of zygosity in transgenik fish is transgenlk achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart.

Transgehik ekspresi transgen yang tinggi pada jaringan ikan F2 yang diperiksa telah diperoleh. Jurnal Akuakultur Indonesia Vol 6, No 2 Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan.

Dengan demikian, metode krt-PCR adalah efektif untuk penentuan sigositas secara cepat dan tepat pada ikan transgenik.